Online MDcat Test Preparation Series Quiz-08

71. Thermus aquatics is the source of _________.

1. Vent polymerase
2. Primase enzyme
3. Taq polymerase
4. Both a and c

72. DNA polymerase III is 

1. Thermostable polymerase
2. Not Thermostable polymerase
3. Both Of The Above
4. None Of These

 

73. Which of the following is the basic requirement of PCR reaction?

1. Two oligonucleotide primers
2. DNA segment to be amplified
3. A heat-stable DNA polymerase
4. All of the above

 

74. Why are vent polymerase and Pfu more efficient than the Taq polymerase?

1. Because of proofreading activity
2. Because of more efficient polymerase activity
3. Both a and b
4. None of the above

 

75. The process of binding of primers to the denatured DNA strands is called?

1. Renaturation
2. Denaturation
3. Annealing
4. None of the above

 

76. Denaturation in PCR is the process Achieved at _________.

1. Heating between 72°C
2. Heating between 40 to 60°C
3. Heating between 90 to 98°C
4. None of the above

 

77. Polymerase used for PCR is extracted from _____________ .

1. Homo sapiens
2. Thermus aquaticus
3. Escherichia coli
4. Saccharomyces cerevisiae

 

78. How many DNA duplexes are obtained from one DNA duplex after 4 cycles of PCR?

1. 8
2. 64
3. 32
4. 16

 

79. The polymer chain reaction is used for_________.

1. Amplifying gene of interest
2. Detecting the presence of the transgene in an organism
3. Constructing RAPD maps
4. All of the above

 

80. The PCR technique was developed by_________.

1. Kohler
2. Kary Mullis
3. Altman
4. Milstein

 

81. First and the most important step in the polymerase chain reaction?

1. Annealing
2. Denaturation
3. Primer extension
4. None of the above

 

82. Which of the following is an application of polymer chain reaction?

1. Site-directed mutagenesis
2. Site-specific recombination
3. Site-specific translocation
4. All of the above

Feedback: When PCR is used for site-directed mutagenesis, the primers are designed to include the desired change, which could be base substitution, addition, or deletion (Figure 1). During PCR, the mutation is incorporated into the amplicon, replacing the original sequence.

 

83. Which of the following is true for asymmetric PCR?

1. Used for generating double-stranded copies for DNA sequence
2. Used for generating single-stranded copies for DNA sequence
3. Both a and b
4. None of the above

Feedback: Asymmetric PCR was used to preferentially amplify the sense strand of the original DNA to a greater extent than the anti-sense strand. Asymmetric PCR is useful in hybridization probing in which only one of the two complementary stands is required. In genetics, a sense strand, or coding strand, is the segment within double-stranded DNA that carries the translatable code in the 5′ to 3′ direction, and which is complementary to the antisense strand of DNA, or template strand, which does not carry the translatable code in the 5′ to 3′ direction.

 

84. Reverse transcription PCR uses ___

1. RNA as a template to form DNA
2. DNA as a template to form ssDNA
3. mRNA as a template to form cDNA
4. All of the above

 

85. DNA polymerase I is a single polypeptide chain with ——- amino acids and molecular weight of 109 kDa. It has three sites.

1. 728 Amino Acids
2. 828 Amino Acids
3. 928 Amino Acids
4. 978 Amino Acids

 

86. DNA polymerase 3 is essential for the replication of the —-

1. leading strands
2. lagging strands
3. Both leading and the lagging strands
4. None of These

 

87. The Polymerase Chain Reaction (PCR) technique, invented in —- by Kary B. Mullis

1. 1885
2. 1985
3. 1995
4. 1988

 

88. DNA polymerase I is an enzyme that participates in the process of prokaryotic DNA replication. Discovered by Arthur Kornberg in

1. 1756
2. 1856
3. 1956
4. 1996

Feedback: DNA polymerase I is an enzyme that participates in the process of prokaryotic DNA replication. Discovered by Arthur Kornberg in 1956, it was the first known DNA polymerase. It was initially characterized in E. coli and is ubiquitous in prokaryotes.

 

89. DNA polymerase I take part in

1. DNA replication
2. DNA repair
3. Both Replication And Repair
4. None Of These

 

90. DNA polymerase 1, 2 and 3 are prokaryotic DNA polymerases involved in DNA replication. Pol 1 catalyzes the repairing of DNA damages. Pol 2 ————. Pol 3 catalyzes the 5′ to 3′ DNA polymerization.

 

1. Catalyzes the fidelity and processivity of DNA replication
2. catalyzes the repairing of DNA damages
3. catalyzes the 5′ to 3′ DNA polymerization
4. None Of These