SOPs To Work In Meidical laboratory

SOPs To Work In medical laboratory

SOPs To Work In laboratory SOPs Stand for standard operating procedures Which we Follow to work in any lab like (Biochemistry / Molecular or Microbiology Etc.) Labs. You Can Complete Prepare yourself For a Lab work With Cluesbook. Visit Lab work and prepare yourself For lab work. Wear lab coat before entering the laboratory and […]

How to Prepare PCI For DNA Extraction

How to Prepare PCI For DNA Extraction

In This Article We Will discus how to Prepare PCI For DNA Extraction For DNA Extraction We Use 25:24:1 Ratio Phenol Chloroform Isoamyl alcohol (PCI)   The magic of PCI in DNA isolation relies on the utilization of chemicals, meaning, how and during which amount you employ the three ingredients. you’ll get excellent results if all […]

How To Prepare Saline Solution For Antibacterial Activity.

How To Prepare Saline Solution For Antibacterial Activity.

Before Discussing How To Prepare Saline Solution For Antibacterial Activity read must Know the development of antibiotics has played a big role in combating the dreaded communicable disease like tuberculosis, pneumonia, typhoid and meningitis in 20th century. However, the improper use of antibiotics led to the event of multidrug resistance (MDR) in microbial flora raising a worldwide public health concern of 21st century. This unforeseen […]

How To Prepare A1/TNE Buffer For DNA Extraction From Blood

How to Prepare TNE Buffer

Recipe to Prepare A1/TNE Buffer for DNA Extraction From Blood How to Prepare TNE Buffer for DNA Extraction From Blood Step by Step Recipe of A1/TNE Buffer for DNA Extraction From Blood Add the following to 800ml of distilled H2O Tris base = 12.1g Sodium EDTA/Na2EDTA.2H2O  =  3.7g Adjust pH to 7.4 with concentrated HCl […]

How To Prepare Potato Dextrose Agar (PDA) Media

How to Prepare Potato dextrose agar

Potato Dextrose Agar, commonly referred to as PDA, is a common bacterial growth medium made with the addition of potatoes and dextrose. It is one of the most widely used media for growing fungi and bacteria. PDA can be supplemented with various acids or antibiotics to prevent the growth of bacteria that can affect yeast […]

How To Prepare DEPC Treated Water In Labs

How to Prepare DEPC Treated Water

How to Prepare DEPC Treated Water In Lab   DEPC Treated Water Recipe Add 1ml of 0.1% Diethylpyrocarbonate (DEPC) to 1000ml distilled water. Mix well and let set at room temperature for 1 hour. Autoclave You can Also Prepare these also How Much NaCl is Required to Prepare 50ml of 6M NaCl Solution.? What is the molarity of […]

How To Prepare 10% SDS Solution For DNA Extraction And Other Lab Usage.

How to prepare 10% SDS Solution For DNA Extraction

Sodium dodecyl sulfate (SDS) is a detergent commonly used in lysis buffers to aid in permeabilising cell membranes and in polyacrylamide gel electrophoresis (SDS-PAGE). SDS  solutions are usually made at 10% and further diluted down to<1% in final solutions as required. How to prepare 10% SDS Solution For DNA Extraction The recipe below is used […]

How To Prepare 0.5M EDTA(Ethylene-Di-Amine-Tetra-Acetic Acid) PH 8.0

How to Prepare 0.5M EDTA

0.5M EDTA Recipe Ethylenediaminetetraacetic-acid is abbreviated as EDTA and can be read as Ethylene-di-amine-tetra-acetic acid. EDTA is a molecule called as chelating agent (A chelating agent is a claw like substance which can get and stick to other molecules. Some types of EDTA stick to Salts like calcium. Other types of EDTA stick to metals Like lead(Pb). We are Going to Prepare […]

How To Prepare 50X TAE Buffer (Tris-Acetate EDTA Buffer)

50X-TAE-Buffer

50X TAE Buffer (Tris-Acetate EDTA buffer) Recipe: Tris-base (MW = 121.14 g/mol)        = 242g 100 % glacial acid                                = 57.1ml 0.5 M EDTA (pH 8.0)                          […]

How to Prepare Lysis Buffer For DNA Extraction From Blood

How to Prepare Lysis Buffer For DNA Extraction From Blood

وَبِالْوَالِدَيْنِ إِحْسَانًا — Treat with kindness your parents (Al-Baqara – 2:83) Here is the Procedure of Making Lysis Buffer for DNA Extraction From Blood. Very simple and single step procedure of making Lysis buffer for DNA Extraction from Blood You can also Check: How to Prepare 0.5M EDTA(Ethylene-di-amine-tetra-acetic Acid) PH 8.0   0.5M EDTA = 2ml 1M HCl = […]

How Much NaCl is Required to Prepare 50ml of 6M NaCl Solution.

How Much NaCl is Required to Prepare 50ml of 6M NaCl Solution.,

Prepare 50ml of 6M NaCl Solution. Method-01 17.532g  NaCl Is Dissolved in 500ml of distilled Water to prepare 500ml NaCl of 6M   We Can also calculate it in another way.   17.532g  NaCl Is Dissolved in 500ml of distilled Water to prepare 500ml NaCl of 6M   After Learning from this article you must […]