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How To Prepare 50X TAE Buffer (Tris-Acetate EDTA Buffer)

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50X TAE Buffer (Tris-Acetate EDTA buffer) Recipe:How To Prepare 50X TAE Buffer

    1. Tris-base (MW = 121.14 g/mol)        = 242g
    2. 100 % glacial acid                                = 57.1ml
    3. 0.5 M EDTA (pH 8.0)                           = 100ml

Add Enough distilled Water to Prepare the 1 Litter Solution.

Procedure:

  1. Weigh 242g Tris-base and dissolve in 700 ml of deionized water.
  2. Add Carefully 57.1 ml of 100 % glacial acetic and 100 ml of 0.5M EDTA (pH 8.0)

Add Enough distilled Water to Prepare the 1 Litter Solution.
Note: The pH of this buffer is not adjusted and should be about 8.5

Storage:
Store stock solution at room temperature

 

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