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How To Prepare Potato Dextrose Agar (PDA) Media

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Potato Dextrose Agar, commonly referred to as PDA, is a common bacterial growth medium made with the addition of potatoes and dextrose. It is one of the most widely used media for growing fungi and bacteria. PDA can be supplemented with various acids or antibiotics to prevent the growth of bacteria that can affect yeast and fungi. Recommendation and classification of yeast bacteria and fungi in milk, cosmetics and clinical samples is recommended. A nutrient-rich base from potato imports promotes fungal production and pigment production in certain dermatophytes while dextrose supports the normal growth of bacteria. Agar is used as a stabilizing agent.

How to Prepare Potato dextrose agar
How to Prepare Potato Dextrose Agar Media

Part (I)

How to Prepare Potato Dextrose Agar.

  1. Take 200g of sliced unpeeled potatoes and Boil in 1 liter of water for 30 minutes.
  2. Use cheesecloth to filter, saving effluent, which is potato infusion.
  3. Add dextrose, agar, and water to effluent. Boil another time to dissolve completely.
  4. Sterilize media by autoclaving at 121ºC and 15psa Pressure for 15 minutes.
  5. Aseptically distribute in sterile Petri dishes.

Part (II)

How to Prepare Potato Dextrose Agar from Commercial Powder. (Already Prepared PDA Powder).

  1. Weigh 39g of commercialized powered and Add to 1 liter of distilled water.
  2. Boil while mixing to dissolve completely.
  3. Sterilize media by autoclaving at 121ºC and 15psa Pressure for 15 minutes.
  4. Aseptically distribute into sterile Petri dishes.

Calculations:

How to Prepare Media in 150ml from Commercial PDA Powder.

As we no that we are taking 39g for 1L. And now we are going to calculate how many grams we take to prepare 150ml media.

We will use the Formula:

Calculations for PDA

Appearance: 

  • Light amber colored clear to slightly opaque gel

PH:

  • PH Should be 5.6 ± 0.2

Storage:

  • Media is both temperature and light sensitive. Store plates away from direct light at a temperature of 2-8ºC. Plates may be used within one week when stored in a clean sterile area. Media should not be used if any signs of contamination, deterioration, and/or expiration date.

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